LAUSR

The main components of the stratum corneum (SC), water, lipids, and proteins, are non‐homogeneously distributed throughout the depth. The quantitative determination of their concentration profiles and penetration depth of topically applied substances are urgent topics of dermatological and cosmetic research. Confocal Raman micro‐spectroscopy has distinct advantages when determining semi‐quantitative concentrations of SC components and topically applied substances non‐invasively and in vivo. In this work, we applied a tailored multivariate curve resolution‐alternating least squares (tMCR‐ALS) method to analyze Raman spectra of the SC in the 2000–4000 cm−1 region for quantitatively determining the concentrations of water, lipids, proteins, and topically applied oils using substance‐related spectral loadings which were allowed to change depth‐dependently from the SC's surface toward its bottom. tMCR‐ALS makes matching of depth‐dependent signal attenuation, that is, the normalization on keratin, unnecessary and requires only a few additional experiments for calibration – Raman spectra of the pure materials and their densities.