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Determining Phosphodiesterase Activity (Radioactive Assay).

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Cyclic-di-GMP phosphodiesterases (PDEs) catalyze the hydrolysis of the bacterial second messenger c-di-GMP. This protocol describes a sensitive radioactive assay for PDE activity in which substrate and product can be quickly… Click to show full abstract

Cyclic-di-GMP phosphodiesterases (PDEs) catalyze the hydrolysis of the bacterial second messenger c-di-GMP. This protocol describes a sensitive radioactive assay for PDE activity in which substrate and product can be quickly and easily separated by thin-layer chromatography.

Keywords: radioactive assay; determining phosphodiesterase; assay; activity radioactive; phosphodiesterase activity

Journal Title: Methods in molecular biology
Year Published: 2017

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