LAUSR

Antibiotics abuse in agricultural products leads to serious food safety issues. To this end, we proposed a mix-and-read and enzyme-free amplified assay for antibiotics based on a dual triple helix-aptamer probe, potentially applicable for on-site monitoring antibiotics residue. Dual triple helix-aptamer probe can leverage the response towards target molecules without enzyme-based amplification, rendering it sensitive and robust for profiling target molecules. The proposed assay allowed to mix-and-read detect chloramphenicol with a detection limit of 0.18 nM. Besides, it accommodated for specifically resolving chloramphenicol among other antibiotics. Chloramphenicol residual in aquatic products in fish and milk can be precisely determined. Thus, the aptamer probe deems to enrich the toolbox for managing antibiotics use.