LAUSR

In this study, to obtain higher agmatine yields using the previously developed E. coli strain AUX4 (JM109 Δ speC Δ speF Δ speB Δ argR), the genes encoding glutamate dehydrogenase ( gdhA), glutamine synthetase ( glnA), phosphoenolpyruvate carboxylase ( ppc), aspartate aminotransferase ( aspC), transhydrogenase ( pntAB), and biosynthetic arginine decarboxylase ( speA) were sequentially overexpressed by replacing their native promoters with the heterologous strong trp, core- trc, or 5P tacs promoters to generate the plasmid-free E. coli strain AUX11. The fermentation results obtained using a 3-L bioreactor showed that AUX11 produced 2.93 g L-1 agmatine with the yield of 0.29 g agmatine g-1 glucose in the batch fermentation, and the fed-batch fermentation of AUX11 allowed the production of 40.43 g L-1 agmatine with the productivity of 1.26 g L-1 h-1 agmatine. The results showed that the engineered E. coli strain AUX11 can be used for the industrial fermentative production of agmatine.