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Abstract In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes… Click to show full abstract
Abstract In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.
Keywords:
trans translation;
green fluorescent;
fluorescent protein;
protein vitro;
reassembling green
Journal Title: Nucleic Acids Research
Year Published: 2020
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Journal Title: Nucleic Acids Research
Year Published: 2020
Link to full text (if available)
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recommendations!