A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
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DOI: 10.1186/s12896-017-0346-5
Abstract: BackgroundRestriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction sites and/or ligation. Here,… read more here.
Keywords: method; gene reconstitution; two single; gene ... See more keywords