LAUSR

Ganoderma is classified as a top grade traditional Chinese medicine for promoting human health by regulating ‘vital energy’. Its potency towards metabolism and energy homeostasis, particularly, metabolic adaptations of adipocytes, needs to be re‐evaluated through an evidence‐based study. Here, the triterpenoid‐rich Ganoderma tsugae ethanol extract (GTEE) was found to contribute towards adipogenesis accompanied with elevated intracellular lipid metabolic flux. Additionally, proteomic profiling revealed GTEE‐upregulated mitochondrial remodeling and chemical energy redox modifications, which display UCP1‐positive browning fat‐selective features and a NADH‐mediated adaptive mechanism. GTEE‐treated mice with diet‐induced obesity also resulted in the amelioration of white adipocyte hypertrophy and the appearance of UCP1‐positive browning adipocytes. Our novel findings unravel that GTEE could promote intracellular metabolic flexibility and plasticity followed by the induction of adipocyte browning.