LAUSR

The MIR449 genomic locus encompasses several regulators of multiciliated cell formation (multiciliogenesis). The miR-449 homologues miR-34b/c represent additional regulators of multiciliogenesis that are transcribed from another locus. Here, we characterized the expression of BTG4, LAYN and HOATZ, located in the MIR34B/C locus using single-cell RNA-seq and super-resolution microscopy from human, mouse or pig multiciliogenesis models. BTG4, LAYN and HOATZ transcripts were expressed in both precursors and mature multiciliated cells. The Layilin/LAYN protein was absent from primary cilia, but it was expressed in apical membrane regions or throughout motile cilia. LAYN silencing altered apical actin cap formation and multiciliogenesis. HOATZ protein was detected in primary cilia or throughout motile cilia. Altogether, our data suggest that the MIR34B/C locus may gather potential actors of multiciliogenesis.