LAUSR

Exploration of clinically acceptable blood glucose monitors has been engaging in the past decades, yet our ability to quantitatively detect blood glucose in a painless, accurate and highly sensitive manner remains limited. Herein, we describe a fluorescence-amplified origami microneedle (FAOM) device that integrates tubular DNA origami nanostructures and glucose oxidase molecules into its inner network to quantitatively monitor blood glucose. Skin-attached FAOM device can collect glucose in situ and transfer the input into proton signal with oxidase's catalysis. The proton-driven mechanical reconfiguration of DNA origami tubes separated fluorescent molecules and their quenchers, eventually amplifying glucose-correlated fluorescence signal. The function equation established on clinical examinees suggested that FAOM can report blood glucose in a highly sensitive and quantitative manner. In clinical blind tests, FAOM achieved well-matched accuracy (98.70 ± 4.77%) compared with commercial blood biochemical analyser and completely meets the requirements of accurately monitoring blood glucose. FAOM device could be inserted into skin tissue in a trivially painful manner and with minimal leakage of DNA origami, substantially improving the tolerance and compliance of blood glucose test. This article is protected by copyright. All rights reserved.