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Aliphatic polyester, poly(3‐hydroxyvalerate) (PHV), is commonly produced as a granular component in bacterial cells of various species. Based on 16S rDNA gene sequence analysis, strain PJC48 was identified as a… Click to show full abstract
Aliphatic polyester, poly(3‐hydroxyvalerate) (PHV), is commonly produced as a granular component in bacterial cells of various species. Based on 16S rDNA gene sequence analysis, strain PJC48 was identified as a Bacillus species. The current study is aimed to screen for a high‐yield strain that can produce PHV efficiently and to increase PHV product yield by optimizing the fermentative process. We identified a high‐producer strain based on Nile red staining. Characterization of the PHV produced by PJC48 by nuclear magnetic resonance spectroscopy revealed that it consisted of (R)‐3‐hydroxyvalerate monomers. The suggested model was validated by response surface methodology. Optimization of the PHV yield resulted in an increase of 32.75% compared to control, with a maximum production of 1.64 g/L after 48 H.
Journal Title: Biotechnology and Applied Biochemistry
Year Published: 2018
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