LAUSR

Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scaleup. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography and CaptoTM Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large-scale production of high-purity Japanese encephalitis virus. This article is protected by copyright. All rights reserved.