LAUSR

Thyroid cancer appears in endocrine glands and specific to thyroid glands, has been reported widely. This work was targeted to identify and quantify thyroglobulin by using anti-thyroglobulin antibody complexed silane surface on Interdigitated electrode (IDE) sensing surface. (3-aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve [y = 0.1311x + 0.5386; R² = 0.9707] with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and non-immune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer. This article is protected by copyright. All rights reserved.