LAUSR

A new glyco-phenol was produced by the coupling between glucosamine (Glu) and ferulic acid (FA) using Myceliophthora thermophila laccase as biocatalyst in mild conditions (distilled water and 30°C) as an environmentally friendly process. Results indicated that the enzymatic reaction created a new derivative (FA-Glu), produced from coupling between Glu and FA by covalent bonds. By the high production of (FA-Glu) derivative and its stability, the optimal ratio of (FA: Glu) was of (1:1) at optimal time reaction of 6 h. Under these optimal conditions, almost 55% of -NH2 groups on Glu were bound with FA-oxidation products. The new derivative showed higher hydrophobic character than Glu due to the presence of FA in its structure. LC-MS analysis showed that (FA-Glu) derivative exhibited a molecular mass at MM 713 g/mol containing one Glu-molecule and three FA-molecules after decarboxylation. Furthermore, the new derivative presented good antioxidant and anti-proliferative activities in comparison with Glu and FA. These results suggest that the enzymatic conjugation between Glu and FA is a promising process to produce a new glyco-phenol having good functional properties for potential applications. This article is protected by copyright. All rights reserved.