LAUSR

Anesthesia-related drugs cause various side effects and health-related illnesses after surgery. In particular, neurogenerative disorder is a common problem of anesthesia-related drugs. A patient undergoes anesthesia as a requirement of the preoperative evaluation to diagnose the medical illness, which is caused by anesthetic drug treatment. Different blood-based biomarkers help to identify the changes appearing in patients after anesthesia treatment. Among them, tau-protein is a sensitive biomarker of neurodegenerative disease, and the fluctuations in tau-proteins are highly associated with various diseases. Furthermore, researchers have found unstable levels of tau-protein after the anesthesia process. The current research was focused on quantifying tau-protein via impedance spectroscopy to identify the problems caused by anesthesia-related drugs. An impedance spectroscopy electrode was modified into a multi-walled carbon nanotube, and an amine-ended aptamer was then attached. This electrode surface was used to quantify the tau-protein level, and reached the detection limit of 1 fM. The determination coefficient was found to be y = 369.93x + 1144.9, with R2 = 0.9846 in the linear range of 1 fM-1 nM. Furthermore, tau-protein spiked human serum was clearly identified on the immobilized aptamer surface, indicating the specific detection. This article is protected by copyright. All rights reserved.