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Square wave voltammetric detection of cholesterol with biosensor based on poly(styrene-co-ε-caprolactone)/MWCNTs composite.

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A novel poly(styrene-co-ε-caprolactone)/multi-walled carbon nanotubes/cholesterol oxidase film coated glassy carbon electrode was designed for cholesterol detection by square wave voltammetry. The biosensor responded to cholesterol with a measurement concentration range… Click to show full abstract

A novel poly(styrene-co-ε-caprolactone)/multi-walled carbon nanotubes/cholesterol oxidase film coated glassy carbon electrode was designed for cholesterol detection by square wave voltammetry. The biosensor responded to cholesterol with a measurement concentration range between 1 μM and 130 μM, a relative standard deviation of only 0.095% and accuracy of 100.42% ±2.85 with the square wave voltammetry technique in the potential range from -0.6 V to +0.6 V. The limit of detection was calculated to be 0.63 μM. The biosensor was preserved 91% and %84 of its initial response at the end of the 9st and 25st days, respectively. Human serum from human male AB plasma was analyzed without pretreatment except for dilution to investigate the performance of the biosensor in a complex medium. This article is protected by copyright. All rights reserved.

Keywords: square wave; poly styrene; styrene caprolactone; detection; cholesterol; biosensor

Journal Title: Biotechnology and applied biochemistry
Year Published: 2022

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