LAUSR

Condensed and hydrolyzable tannins are secondary metabolites present in almost every plant part.Tannase enzyme acts on hydrolyzable tannins to produce gallic acid and tannase- mediated end-products with immense therapeutic potential. Seven different fruits with significant presence of hydrolysable tannin content were selected to check for phenol, tannin and hydrolyzable tannin contents. P. domestica had the maximum phenol content i.e., 85.4±0.207, followed by S. cumini, F. ananassa, R. fruticosus and P. guajava. Plum showed the maximum number of hydrolyzable tannins. Fruit extracts were subjected to tannase hydrolysis and their anti-microbial and anti-oxidant activities were determined. There was a significant increase in the anti-oxidant abilities of the fruits with P. granatum extract, displaying the highest decline of 132 units of IC50 followed by F. ananassa hydrolyzable extract, showing a decrease from 224.75 μg/ml to 119.98 μg/ml. The extracts also depicted a significant increase in anti-bacterial activity after hydrolysis against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus with Rubus idaeus aqueous extract observed to be most effective against E.coli. The increase in anti-oxidant and anti-bacterial activity can be attributed to the production of Tannase mediated products formed after the biotransformation of hydrolyzable tannins present in the aqueous extracts. This article is protected by copyright. All rights reserved.