LAUSR

Dengue fever, a mosquito‐borne viral infection, poses a significant global health challenge, particularly in tropical and subtropical regions. The absence of non‐effective vaccines and specific treatments underscores the need for advanced diagnostic tools for early detection and management. This study presents a novel biosensor for detecting dengue virus type 4 (DENV‐4) by combining carbonyldiimidazole nanoflower (CDI‐NF) with Mn3O4 on laser‐scribed graphene (LSG). Material characterization techniques, including Raman spectroscopy, TEM, XRD, XPS, and FTIR, were employed to confirm the successful integration of Mn3O4 and CDI‐NF, resulting in a unique 3D flower‐like structure. In order to verify the sensing efficiency, a selective DNA sample captured on LSG/Mn3O4‐CDI‐NF was investigated for specifc binding with Aedes aegypti target DNA through selective hybridization and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1 fM, where the sensitivity was confirmed by large transfer resistance (Rct) before and after hybridization with a regression coefficient 0.97373. EIS results demonstrated successful surface modifications and the biosensor's specificity in distinguishing between complementary, mismatched, and non‐complementary target sequences. The biosensor's ability to differentiate between these sequences highlights its potential for accurate and targeted DENV‐4 detection, offering a promising avenue for advancing dengue diagnostics.