LAUSR

BACKGROUND Prenatal ethanol (EtOH) exposure is associated with adverse effect on the male reproductive function. Dietary docosahexaenoic acid (DHA) is known to improve testis function and sperm parameters, thereby male fertility. This study piloted whether dietary DHA influences testis development and function in rats exposed to prenatal EtOH. METHODS Pregnant female Sprague-Dawley rats (n = 30) received either EtOH (3 g/kg, twice a day, n = 14) or dextrose (n = 16) throughout pregnancy. Moreover, they were fed either diet supplemented with (Cont + DHA, n = 8, EtOH + DHA, n = 6) or without DHA (1.4% w/w of total fatty acids) (Cont, EtOH, n = 8 each), with pups being continued on their mothers' diet after weaning. Tissues were collected at gestational day (GD) 20, postnatal day (PD) 4, 21, 49 and 90 for analyzing testicular developmental markers and sperm parameters, and plasma for testosterone. RESULTS Dietary DHA increased serum testosterone at GD20 (p < .05) and sperm normal morphology at PD90 (p < .0001) compared to the group without DHA supplementation. Dietary DHA also increased the height of germinal epithelium at peripuberty, PD49 (p < .03). The EtOH exposure induced a marked decline in the testicular gene expression of StAR at PD49 (p < .02) than those of non-EtOH treated group. CONCLUSIONS These findings indicate that dietary DHA may positively contribute to male fertility by impacting sperm normal morphology likely by increasing fetal testosterone level. Prenatal EtOH exposure did not adversely affect the overall testis developmental markers during development and sperm parameters in adulthood.