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Advancing a rapid, high throughput screening platform for optimization of lentivirus production

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Lentiviral vectors (LVVs) hold great promise as delivery tools for gene therapy and chimeric antigen receptor T cell (CAR‐T) therapy. Their ability to target difficult to transfect cells and deliver… Click to show full abstract

Lentiviral vectors (LVVs) hold great promise as delivery tools for gene therapy and chimeric antigen receptor T cell (CAR‐T) therapy. Their ability to target difficult to transfect cells and deliver genetic payloads that integrate into the host genome makes them ideal delivery candidates. However, several challenges remain to be addressed before LVVs are more widely used as therapeutics including low viral vector concentrations and the absence of suitable scale‐up methods for large‐scale production. To address these challenges, we have developed a high throughput microscale HEK293 suspension culture platform that enables rapid screening of conditions for improving LVV productivity.

Keywords: production; rapid high; high throughput; advancing rapid; throughput screening

Journal Title: Biotechnology Journal
Year Published: 2021

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