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Labour‐saving construction of a target protein interaction network by selective culture and high‐throughput sequencing

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Yeast two‐hybrid (Y2H) is a classic method of screening for protein‐protein interactions. However, the operation process is labor intensive and time consuming, and there is a high possibility of false… Click to show full abstract

Yeast two‐hybrid (Y2H) is a classic method of screening for protein‐protein interactions. However, the operation process is labor intensive and time consuming, and there is a high possibility of false positives and false negatives.

Keywords: protein; protein interaction; target protein; construction target; saving construction; labour saving

Journal Title: Biotechnology Journal
Year Published: 2021

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