LAUSR

BACKGROUND There has been an increasing demand for optically pure D-lactic and L-lactic acid for the production of stereocomplex-type polylactic acid. The D-lactic acid production from lignocellulosic biomass is important owing to its great abundance in nature. Corn steep liquor (CSL) is a cheap nitrogen source used for industrial fermentation, though it contains a significant amount of L-lactic acid, which decreases the optical purity of D-lactic acid produced. METHOD AND RESULTS To remove L-lactic acid derived from the CSL-based medium, L-lactate oxidase (LoxL) from Enterococcus sp. NBRC 3427 was expressed in an engineered Lactiplantibacillus plantarum (formally called Lactobacillus plantarum) strain KOLP7, which exclusively produces D-lactic acid from both hexose and pentose sugars. When the resulting strain was applied for D-lactic acid fermentation from the mixed sugars consisting of the major constituent sugars of lignocellulose (35 g/L glucose, 10 g/L xylose, and 5 g/L arabinose) using the medium containing 10 g/L CSL, it completely removed L-lactic acid derived from CSL (0.52 g/L) and produced 41.7 g/L of D-lactic acid. The L-lactic acid concentration was below the detection limit, and improvement in the optical purity of D-lactic acid was observed (from 98.2% to > 99.99%) by the overexpression of LoxL. CONCLUSION AND IMPLICATIONS The LoxL-mediated consumption of L-lactic acid would enable the production of optically pure D-lactic acid in any medium contaminated by L-lactic acid. This article is protected by copyright. All rights reserved.