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Flow cytometry is a powerful tool for identifying and quantifying various cell markers, such as viability, vitality, and individual cell age, at single‐cell stages. However, cell autofluorescence and marker fluorophore… Click to show full abstract
Flow cytometry is a powerful tool for identifying and quantifying various cell markers, such as viability, vitality, and individual cell age, at single‐cell stages. However, cell autofluorescence and marker fluorophore signals overlap at low fluorescence intensities. Thus, these signals must be unmixed before determining the age fraction.
Keywords:
fluorescence unmixing;
prediction model;
age;
autofluorescence prediction;
model fluorescence;
fluorescence
Journal Title: Biotechnology Journal
Year Published: 2022
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Journal Title: Biotechnology Journal
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!