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Post‐refolding stability considerations for optimization of in‐vitro refolding: L‐asparaginase as a case study

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L‐Asparaginase is an essential enzyme for the food and biopharmaceutical industry. The stability, however, of L‐asparaginase is widely known to be an issue. Commercial manufacturing of any biopharmaceutical involves hold‐ups… Click to show full abstract

L‐Asparaginase is an essential enzyme for the food and biopharmaceutical industry. The stability, however, of L‐asparaginase is widely known to be an issue. Commercial manufacturing of any biopharmaceutical involves hold‐ups during processing, and can result in product loss if stability is an issue, as is the case with L‐asparaginase. This interplay of product intermediate stability and process design is the focus of this investigation.

Keywords: post refolding; stability considerations; asparaginase; case; refolding stability; stability

Journal Title: Biotechnology Journal
Year Published: 2022

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