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Optimizing VLP-production in Gene therapy: Opportunities and challenges for in silico modeling.

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Over the past decades, virus-like particle (VLP)-based gene therapy (GT) evolved as a promising approach to cure inherited diseases or cancer. Tremendous costs due to inefficient production processes remain one… Click to show full abstract

Over the past decades, virus-like particle (VLP)-based gene therapy (GT) evolved as a promising approach to cure inherited diseases or cancer. Tremendous costs due to inefficient production processes remain one of the key challenges despite considerable efforts to improve titers. This review aims to link genome-scale metabolic models (GSMMs) to cell lines used for VLP synthesis for the first time. We summarize recent advances and challenges of GSMMs for Chinese hamster ovary (CHO) cells and provide an overview of potential cell lines used in GT. Although GSMMs in CHO cells led to significant improvements in growth rates and --1 production, no GSMM has been established for VLP production so far. To facilitate the generation of GSMMs for these cell lines we further provide an overview of existing omics data and the highest production titers so far reported. This article is protected by copyright. All rights reserved.

Keywords: vlp production; production; gene therapy; cell lines

Journal Title: Biotechnology journal
Year Published: 2023

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