LAUSR

In spite of tremendous efforts dedicated to addressing bacterial infections and biofilm formation, the post‐antibiotic ear continues to witness a gap between the established materials and an easily accessible yet biocompatible antibacterial reagent. Here we show carbon dots (CDs) synthesized via a single hydrothermal process can afford promising antibacterial activity that can be further enhanced by exposure to light. By using citric acid and polyethyleneimine as the precursors, the photoluminescence CDs can be produced within a one‐pot, one‐step hydrothermal reaction in only 2 h. The CDs demonstrate robust antibacterial properties against both Gram‐positive and Gram‐negative bacteria and, notably, a considerable enhancement of antibacterial effect can be observed upon photo‐irradiation. Mechanistic insights reveal that the CDs generate singlet oxygen (1O2) when exposed to light, leading to an augmented reactive oxygen species level. The approach for disruption of biofilms and inhibition of biofilm formation by using the CDs has also been established. Our findings present a potential solution to combat antibacterial resistance and offer a path to reduce dependence on traditional antibiotics.