D‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed… Click to show full abstract
D‐Allulose is one of the most well‐known rare sugars widely used in food, cosmetics, and pharmaceutical industries. The most popular method for D‐allulose production is the conversion from D‐fructose catalyzed by D‐allulose 3‐epimerase (DAEase). To address the general problem of low catalytic efficiency and poor thermostability of wild‐type DAEase, D‐allulose biosensor was adopted in this study to develop a convenient and efficient method for high‐throughput screening of DAEase variants.
Keywords:
thermostability;
semi rational;
engineering allulose;
allulose epimerase;
allulose biosensor;
rational engineering
Journal Title: Biotechnology Journal
Year Published: 2024
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Journal Title: Biotechnology Journal
Year Published: 2024
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!