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5‐Methylcytidine (m5C) and 5‐methyluridine (m5U) are highly abundant post‐transcriptionally modified nucleotides that are observed in various natural RNAs. Such nucleotides were labeled through a chemical approach, as both underwent oxidation… Click to show full abstract
5‐Methylcytidine (m5C) and 5‐methyluridine (m5U) are highly abundant post‐transcriptionally modified nucleotides that are observed in various natural RNAs. Such nucleotides were labeled through a chemical approach, as both underwent oxidation at the C5=C6 double bond, leading to the formation of osmium–bipyridine complexes, which could be identified by mass spectrometry. This osmium tag made it possible to distinguished m5C and m5U from their isomers, 2′‐O‐methylcytidine and 2′‐O‐methyluridine, respectively. Queuosine and 2‐methylthio‐N6‐isopentenyladenosine in tRNA were also tagged through complex formation.
Journal Title: ChemBioChem
Year Published: 2018
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