LAUSR

Pi‐class glutathione S‐transferase (GSTP1) is highly expressed in a wide variety of human cancer tissues compared to the corresponding normal counterpart. Therefore, GSTP1 is a potential target enzyme for overcoming resistance to chemotherapeutic agents or visualizing specific lesions such as cancer. Here, we present orange and red fluorescence‐emitting probes selective for GSTP1. Carbofluorescein and TokyoMagenta fluorophores were modified with a previously described GSTP1‐selective chromogenic compound to generate orange and red fluorescence probes, respectively. Of these probes, Ps‐CF, the orange fluorescence‐emitting probe, was confirmed to be highly specific for detecting GSTP1 exogenously or endogenously expressed in various cancer cells. Additionally, it was demonstrated that Ps‐CF is applicable for the simultaneous detection of GSTP1 and another cancer‐associated enzyme by using a green fluorescence emitting γ‐glutamyl transpeptidase (GGT) probe. In conclusion, the fluorescent probes developed in this study enable the simultaneous detection of multiple tumour markers such as GSTP1 with other cancer‐associated enzymes by concurrently using spectrally distinguished fluorescent probes, potentially broadening the scope of cancer detection.