This study examined how manual temperature control affects the separation of two enantiomers of five racemic mixtures using high-performance liquid chromatography (HPLC) in terms of separation factor (α), resolution (Rs),… Click to show full abstract
This study examined how manual temperature control affects the separation of two enantiomers of five racemic mixtures using high-performance liquid chromatography (HPLC) in terms of separation factor (α), resolution (Rs), and the number of theoretical plates (N). The results showed that heating/cooling during one time HPLC analysis improved the separation factor and resolution.
               
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