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Improvement of chiral separation efficiency through temperature control during one time high performance liquid chromatography analysis.

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This study examined how manual temperature control affects the separation of two enantiomers of five racemic mixtures using high-performance liquid chromatography (HPLC) in terms of separation factor (α), resolution (Rs),… Click to show full abstract

This study examined how manual temperature control affects the separation of two enantiomers of five racemic mixtures using high-performance liquid chromatography (HPLC) in terms of separation factor (α), resolution (Rs), and the number of theoretical plates (N). The results showed that heating/cooling during one time HPLC analysis improved the separation factor and resolution.

Keywords: performance liquid; one time; liquid chromatography; temperature control; high performance; separation

Journal Title: Chirality
Year Published: 2022

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