LAUSR

Rho‐GTPase‐activating proteins (Rho‐GAPs) are essential upstream regulators of the Rho family of GTPases. Currently, it remains unclear if the phenotypic change caused by perturbations to a Rho‐GAP is predictable from its amino acid sequence. Here we analyze the relationship between the morphological response of cells to the silencing of Rho‐GAPs and their primary structure. For all possible pairs of 57 different Rho‐GAPs expressed in MCF10A epithelial cells, the similarity in the Rho‐GAP silencing‐induced morphological change was quantified and compared to the similarity in the primary structure of the corresponding pairs. We found a distinct correlation between the morphological and sequence similarities in a specific group of RhoA‐targeting Rho‐GAPs. Thus, the family‐wide analysis revealed a common feature shared by the specific Rho‐GAPs.