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Deep targeted sequencing of cytological tumor cells using whole genome amplification

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Genomic profiling in lung cancer is essential for precision medicine. Cytological specimens provide an alternative to formalin‐fixed paraffin‐embedded (FFPE) samples for comprehensive genomic analysis. However, this approach remains challenging when… Click to show full abstract

Genomic profiling in lung cancer is essential for precision medicine. Cytological specimens provide an alternative to formalin‐fixed paraffin‐embedded (FFPE) samples for comprehensive genomic analysis. However, this approach remains challenging when a limited number of tumor cells are available. We applied whole genome amplification (WGA) to cytology specimens to overcome this limitation.

Keywords: whole genome; genome amplification; tumor cells; deep targeted

Journal Title: Cancer Cytopathology
Year Published: 2022

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