LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Preparation and Co‐Culture of iPSC‐Derived Dopaminergic Neurons and Astrocytes

Photo from wikipedia

Induced pluripotent stem cell (iPSC)‐based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient‐derived neurons and astrocytes allows investigation of the molecular mechanisms… Click to show full abstract

Induced pluripotent stem cell (iPSC)‐based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient‐derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and development. In particular, these two cell types can be mono‐ or co‐cultured to study the influence of cell‐autonomous and non‐cell‐autonomous contributors to neurodegenerative diseases. We developed a streamlined procedure to produce high‐quality/high‐purity cultures of dopaminergic neurons and astrocytes that originate from the same population of midbrain floor‐plate progenitors. This unit describes differentiation, quality control, culture parameters, and troubleshooting tips to ensure the highest quality and reproducibility of research results. © 2019 The Authors.

Keywords: dopaminergic neurons; preparation culture; ipsc; neurons astrocytes; cell

Journal Title: Current Protocols in Cell Biology
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.