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In‐cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells… Click to show full abstract
In‐cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near‐to‐atomic resolution. In this article, detailed instructions are described for setting up an in‐cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step‐by‐step protocols for both the preparation of an in‐cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in‐cell NMR experiments are discussed. © 2018 by John Wiley & Sons, Inc.
Journal Title: Current Protocols in Nucleic Acid Chemistry
Year Published: 2019
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