LAUSR

Inspired by water-forming NAD(P)H oxidases, a cooperative photobiocatalytic system was designed to aerobically regenerate the oxidized nicotinamide cofactors in this work. Photocatalysts enabled NAD(P)H oxidation to the oxidized forms with O 2 under irradiation of visible light, producing H 2 O 2 as byproduct, which was subsequently used as oxidant by horseradish peroxidase-mediator system (PMS) to oxidize NAD(P)H. The photobiocatalytic system showed a turnover frequency of 8 800 min -1 in the oxidation of NAD(P)H. Photobiocatalytic NAD(P)H oxidation could smoothly proceed within pH 6-9. In addition to natural NAD(P)H, the synthetic biomimetics were good substrates of this regeneration system. Total turnover numbers up to 180 000 for cofactor were obtained when the photobiocatalytic regeneration system was coupled with dehydrogenase-catalytic oxidations. It may be a promising protocol to recycle the oxidized cofactors for catalytic oxidations.