LAUSR

This 28-color flow cytometry panel was developed to detect frequencies and function of a broad spectrum of T-cell subsets from the blood of infants and adults following antigen-specific stimulation. This panel includes markers to characterize αβ and γδ T-cell subsets, T regulatory cells (Tregs), T follicular helper cells (TfH), and recent thymic emigrants (RTEs). Additionally, antibodies that measure cell surface activation molecules (CD154, CD69, HLA-DR, and CD38) and function through detection of cytokines (IL-8, IL-2, IL-4, IL-5, IL-13, IL-21, IFNγ, and TNF) are also included. This panel was optimized using PMA-stimulated, cryopreserved healthy adult and infant PBMCs as well as stimulation with CMV-specific peptides. Currently, this panel is being used to evaluate antigen-specific T-cell responses in adults and infants following immunization with an attenuated malaria vaccine (Table 1).