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Preliminary report on monolayer preparation of cervical sample by indigenous technique

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The conventional Papanicoloaou's (PAP) smears are often inadequate and tedious to report. There are various commercially available techniques to process monolayer cervical smears. These commercially available techniques are expensive and… Click to show full abstract

The conventional Papanicoloaou's (PAP) smears are often inadequate and tedious to report. There are various commercially available techniques to process monolayer cervical smears. These commercially available techniques are expensive and cannot be installed in primary health centres. Herein, we attempted to prepare modified Millipore technique to make the monolayer cervical smear. We compared both the modified Millipore technique (MMT) and liquid-based cytology (LBC) by SurePath to assess the effectiveness of the MMT. It was a prospective study. The sample processing was performed after taking prior consent from each patient, and the identity of the patient was not revealed. The study followed the Helsinki accord and ethics were maintained. The present study was made in 30 cases of cervical samples. The cervical sample was collected in BD sure path vial with rovers' cervix brush. The brush was dropped into the vial itself after the collection of the sample. The LBC preparations and MMT smears were compared by the competent cytopathologists (PD). The parameters which were studied were as follows:

Keywords: sample; technique; cervical sample; monolayer; preliminary report

Journal Title: Diagnostic Cytopathology
Year Published: 2020

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