Fig. 1. Overview of transgenic constructs. A: Myc-tagged Cdc42 and RhoA constructs are placed downstream of mCherry and the self-cleaving viral peptide f2a, enabling their bicistronic expression following activation of… Click to show full abstract
Fig. 1. Overview of transgenic constructs. A: Myc-tagged Cdc42 and RhoA constructs are placed downstream of mCherry and the self-cleaving viral peptide f2a, enabling their bicistronic expression following activation of the 10xuas promoter. 10xuas activation leads to the bicistronic expression of egfp-f2a and Rac1. Each construct includes cmlc2:egfp oriented in reverse orientation B: Chart detailing each specific isoform of Rho GTPase, with its predicted activity and species of origin.
               
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