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The most‐common strategy for zebrafish Cre/lox‐mediated lineage labeling experiments combines ubiquitously expressed, lox‐based Switch reporter transgenes with tissue‐specific Cre or 4‐OH‐Tamoxifen‐inducible CreERT2 driver lines. Although numerous Cre driver lines have… Click to show full abstract
The most‐common strategy for zebrafish Cre/lox‐mediated lineage labeling experiments combines ubiquitously expressed, lox‐based Switch reporter transgenes with tissue‐specific Cre or 4‐OH‐Tamoxifen‐inducible CreERT2 driver lines. Although numerous Cre driver lines have been produced, only a few broadly expressed Switch reporters exist in zebrafish and their generation by random transgene integration has been challenging due to position‐effect sensitivity of the lox‐flanked recombination cassettes. Here, we compare commonly used Switch reporter lines for their recombination efficiency and reporter expression pattern during zebrafish development.
Journal Title: Developmental Dynamics
Year Published: 2022
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