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Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique‐sensitive and time‐consuming. Thus, we utilized an existing model… Click to show full abstract
Recent advances in tissue regeneration approaches including 3D organoids, were based on various 3D organogenesis models. However, 3D models are generally technique‐sensitive and time‐consuming. Thus, we utilized an existing model of submandibular salivary gland (SMG) to modify a simple and highly reproducible in vitro 3D culture model of primary SMG cells self‐organization into a well‐developed cell spheroid inside Matrigel substrate. We used this model to observe the collective multicellular behavior during spheroid formation. Further, we applied various quantitative approaches including real‐time live imaging and immune histochemical image analysis to dissect the cellular dynamics during tissue patterning.
Journal Title: Developmental Dynamics
Year Published: 2019
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