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Figure 1. Synopsis of the experimental strategy. The CD21-positive, human leukocyte antigen (HLA) class II-negative lymphoblastoid cell line 721.174 was co-transfected with plasmids encoding the HLA-DR α-chain HLA-DRA*01:01 and the… Click to show full abstract
Figure 1. Synopsis of the experimental strategy. The CD21-positive, human leukocyte antigen (HLA) class II-negative lymphoblastoid cell line 721.174 was co-transfected with plasmids encoding the HLA-DR α-chain HLA-DRA*01:01 and the HLA-DR β-chain HLA-DRB1*01:01 or HLADRB1*15:01. After 1 day, the transfected cells were infected by spinoculation with recombinant EBV containing the gene for GFP (EBV-GFP). Three days later, the percentage of EBV-GFP infected among HLA-DRB1 transfected cells was determined by flow cytometry.
Journal Title: European Journal of Immunology
Year Published: 2021
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