LAUSR

Nowadays laparoscopic interventions enable the collection of resident macrophage populations out of the human cavities. We employed this technique to isolate pleural monocytes/macrophages from healthy young adults who underwent a correction of pectus excavatum. High quality CD14+ monocytes/macrophages (plMo/Mφ) were used for RNA‐sequencing (RNA‐seq) in comparison with human monocyte‐derived macrophages (MDM) natural (MDM‐0) or IL‐4‐polarized (MDM‐IL4). Transcriptome analysis revealed 7166 and 7076 differentially expressed genes (DEGs) in plMo/Mφ relative to natural MDM‐0 and polarized MDM‐IL4, respectively. The gene set enrichment analysis, which was used to compare RNA‐seq data from plMo/Mφ with single‐cell (scRNA‐seq) data online from human bronchial lavage macrophages, showed that plMo/Mφs are characterized by a high expression of genes belonging to the metallothionein (MT) family, and that the expression of these genes is significantly higher in plMo/Mφ than in MDM‐0 or MDM‐IL4. Our results provide additional insights on high MTs‐expressing macrophage subsets, which seem to be present not only in bronchial lavage of healthy adults or in pleural exudates of lung cancer patients but also in pleural fluid of healthy young adults. Macrophage subsets expressing high MTs may have specific roles in lung defense, repair, and homeostasis, and require further investigations.