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Efficient Expression and Purification of Recombinant Mouse Dimeric IgA

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and… Click to show full abstract

Immunoglobulin (Ig) A is the main antibody isotype found on mucosal surfaces in mammals, where it is predominantly present as a dimer. Here we provide an easy, scalable, efficient, and broadly applicable method to produce and purify monoclonal mouse dimeric IgA from single B cell Ig transcripts to study mucosal antibody responses at single‐cell level.

Keywords: efficient expression; expression purification; mouse dimeric; dimeric iga

Journal Title: European Journal of Immunology
Year Published: 2025

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