LAUSR

The rapidly growing, competitive biopharmaceutical market requires tight bioprocess monitoring. An integrated, automated platform for the routine on-line/ at-line monitoring of key factors in the cell culture medium could greatly improve process monitoring. Mono- and disaccharides, as the main energy and carbon source, are one of these key factors. A CE-LIF method was developed for the analysis of several mono- and disaccharides, considering requirements and restrictions for analysis in an integrated, automated monitoring platform, such as the possibility for miniaturisation to microchip electrophoresis. Analysis was performed after fluorescent derivatisation with 8-aminopyrene-1,3,6-trisulfonic acid (APTS). The derivatisation reaction and the separation BGE were optimised using Design of Experiments. The developed method is applicable to the complex matrix of cell culture medium and proved transferable to microchip electrophoresis. This article is protected by copyright. All rights reserved.