LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Investigating peak dispersion in free‐flow counterflow gradient focusing due to electroosmotic flow

Photo by codioful from unsplash

Free‐flow electrophoresis (FFE) has the ability to continuously separate charged solutes from complex biological mixtures. Recently, a free‐flow counterflow gradient focusing mechanism has been introduced to FFE, and it offers… Click to show full abstract

Free‐flow electrophoresis (FFE) has the ability to continuously separate charged solutes from complex biological mixtures. Recently, a free‐flow counterflow gradient focusing mechanism has been introduced to FFE, and it offers the potential for improved resolution and versatility. However, further investigation is needed to understand the solute dispersion at the focal position. Therefore, the goal of this work is to model the impact of electroosmotic flow, which is found to produce a pressure‐driven backflow to maintain the fixed counterflow inputs. Like the counterflow, this backflow has a parabolic velocity profile that must be considered when predicting the concentration distribution of a given solute. After the model is established, preliminary experimental results are presented for a qualitative comparison. Results demonstrate a reasonable agreement at low applied voltages and provide a strong framework for future experimental validation.

Keywords: gradient focusing; flow counterflow; free flow; flow; counterflow gradient

Journal Title: ELECTROPHORESIS
Year Published: 2022

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.