LAUSR

In order to accelerate SDS-PAGE, here we propose an optimized version of the technique enabled by experimental tuning reinforced by theoretical description. In the resulting system, the gel buffer was diluted two-fold and supplemented with glycine at a low concentration, while a higher voltage was applied. This approach reduced runtime from 90 to 18 minutes. It is important to emphasize that, despite the high voltage applied to the gel, the resolution of the bands did not decrease compared to the original Laemmli method. The proposed acceleration approach can be used in other variants of SDS-PAGE. This article is protected by copyright. All rights reserved.