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Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

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Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the… Click to show full abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

Keywords: towards electroenzymatic; process; old yellow; electroenzymatic processes; processes involving; yellow enzymes

Journal Title: Engineering in Life Sciences
Year Published: 2017

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