LAUSR

CRISPR/Cas9‐mediated targeted gene integration (TI) has been used to generate recombinant mammalian cell lines with predictable transgene expression. Identifying genomic hot spots that render high and stable transgene expression and knock‐in (KI) efficiency is critical for fully implementing TI‐mediated cell line development (CLD); however, such identification is cumbersome. In this study, we developed an artificial KI construct that can be used as a hot spot at different genomic loci. The ubiquitous chromatin opening element (UCOE) was employed because of its ability to open chromatin and enable stable and site‐independent transgene expression. UCOE KI cassettes were randomly integrated into CHO‐K1 and HEK293T cells, followed by TI of enhanced green fluorescent protein (EGFP) onto the artificial UCOE KI site. The CHO‐K1 random pool harboring 5′2.2A2UCOE‐CMV displayed a significant increase in EGFP expression level and KI efficiency compared with that of the control without UCOE. In addition, 5′2.2A2UCOE‐CMV showed improved Cas9 accessibility in the HEK293T genome, leading to an increase in indel frequency and homology‐independent KI. Overall, this assessment revealed the potential of UCOE KI constructs as artificial integration sites in streamlining the screening of high‐production targeted integrants by mitigating the selection of genomic hot spots.