LAUSR

Siloxanes are used in personal care, biomedical, and industrial products. Their worldwide use and persistence in the environment cause consistent exposure for both humans and aquatic animals. Two siloxane congeners, decamethylcyclopentasiloxane (D5; CAS 541‐02‐6) and octamethylcyclotetrasiloxane (D4; CAS 556‐67‐2), are among the most prevalent, with measurable levels in air, sediment, water, and biological samples. However, few studies have examined the impact of developmental (embryo/larva) exposure. To address this gap, we performed parallel experiments using wildtype zebrafish (Danio rerio). One set of experiments used laboratory‐mixed individual solutions containing either D4, D5, or 2,4,6,8‐tetramethylcyclotetrasiloxane (D4H; CAS 2370‐88‐9); the other used environmental water samples containing a mixture of siloxanes, including D4 and D5. These samples were collected from Bladensburg Waterfront Park (BWP) a site along the Anacostia River, Washington, DC. In both experiments, zebrafish (24–48 h postfertilization, hpf) were exposed until 7 or 14 days (d)pf. Chronic exposure to D4, D5, or BWP water until 7 dpf caused stress‐like behaviors and reduced swim velocities; anatomical differences were noted only in BWP‐exposed larvae. At 14 dpf, BWP‐treated larvae still showed slower swimming velocities and increased immobility; anatomical differences were no longer evident and thigmotactic behavior was reduced. D4 and D5‐exposed larvae did not survive after 10 dpf. Larvae exposed to D4H showed no decreases in behavior or growth at either age. These results suggest early developmental sensitivity to siloxane exposure and point to the need to consider embryonic/larval endpoints when assessing aquatic contaminants.