LAUSR

This study is aimed to find an approach for effective skin optical clearing in vivo using polyethylene glycol 300 (PEG-300) as an optical clearing agent in combination with physical enhancers: fractional laser microablation (FLMA), and/or low-frequency sonophoresis. In this study albino outbred rats were used. Light attenuation coefficient and optical clearing potential of these approaches were evaluated in upper (from ~70 to ~200 μm) and middle (from ~200 to ~400 μm) dermis separately using optical coherence tomography (OCT). In 30 min, optical clearing potential of sonophoresis in combination with FLMA and PEG-300 in the upper dermis was the maximal (2.3 ± 0.4) in comparison with other treatments in this time point. The most effective approach for optical clearing of middle dermis was PEG-300 and sonophoresis; but the maximal value of optical clearing potential (1.6 ± 0.1) was achieved only in 90 min. Figure: OCT image of rat skin in vivo after fractional laser microablation. Arrow points to microchannel. Bars correspond to 100 μm. This article is protected by copyright. All rights reserved.