LAUSR

Photobiomodulation-Therapy (PBMT) is known as a complementary tool to alleviate pain sensation in patients, nevertheless, there is still a gap of knowledge on its mechanism of action, thus limiting its clinical employment. In this study a possible molecular mechanism of the 905 nm PBMT (0.25 W/cm2 ;3,6,12,18 J/cm2 ,5 Hz) analgesic effect was tested on 50B11 cells, by investigating its impact on mitochondria. A decrement of ATP was detected, moreover, an increment of total ROS and mitochondrial superoxide anion was found after PBMT with all protocols tested. PBMT at 18j diminished the mitochondrial membrane potential, and influenced mitochondrial respiration, decreasing the oxygen consumption rate. Finally, a decrement of ERK1/2 phosphorylation was observed with the protocol using 12j. Taken together these findings highlighted the intracellular effects, mainly correlated to mitochondrial, induced by 905 nm PBMT in sensory neurons, indicating the central role of this organelle in the cellular response to 905 nm near-infrared laser light. This article is protected by copyright. All rights reserved.